Three varieties used for wine production were utilized in the present study, namely ‘Catarratto’, ‘Frappato’, and ‘Nero d’Avola’ [45,46]. The plant material was collected from the germplasm repository for perennial plants at the Institute of Biosciences and BioResources of the National Research Council of Italy (CNR-IBBR) located in Collesano district (province of Palermo), Italy (37°59′19.9″ N, 13°54′55.8″ E, 80 m a.s.l.). Different floral explants (pistil, anther/filament, and ovary) used for culture initiation were dissected from flowers harvested 15 days before anthesis.

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