IC50 and EC50 values were determined by nonlinear regression analysis of concentration response curves using GraphPad Prism 6.0 (GraphPad Software, La Jolla, CA). For tumor studies, mean tumor volume and standard error shown were analyzed using the Student’s t-test for differences in T/C values (StatView, SAS Institute, Cary, NC). For the PDX N = 3 screening study, the selectivity and anti-tumor activity efficacy of ABBV-176 was by the Welch t-test correlating the log transformed relative tumor volume at the time of maximum tumor growth inhibition (TGI), and mixed effect modeling, comparing the log of fold change in tumor volume (versus the first time point) for every available time point using R software (Bell Laboratories, Madison, WI). Both tests were used, with ≥1 significant test describing treatment efficacy. Survival curves were generated and analyzed using the JMP statistical software v7 (SAS institute, Cary, NC). The Log-Rank (Mantle-Cox) test was used to calculate the survival improvement of treatments.

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