Two to three replicate untreated tumors were subjected to Western blot analysis as previously described [24] with anti-PRLR (inv359200, Invitrogen) and anti-actin (Sigma, # A5441). To assess relative PRLR expression levels for xenograft lysates, the low PRLR-expressing MCF7 xenograft (~ 8000 receptors/cell in culture) lysates were run in parallel as an internal standard. For each individual MCF7 lysate, the total PRLR signal was divided by the actin signal and the average value then used to normalize the other CDX and PDX lysates relative to the MCF7 PRLR value.

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