The luciferase assay was performed with a Dual-Luciferase® Reporter Assay System (E1960, Promega) according to the manufacturer’s instructions. Briefly, growth medium was removed from the cultured cells in a 12-well culture plate, and a sufficient volume of 1 × phosphate-buffered saline (PBS) was gently applied to rinse the bottom of the culture vessel. After 250 μL of 1 × Passive Lysis Buffer (PLB) was added to the wells, the plate was shaken gently at room temperature for 15 min, and the cell lysate was transferred to a white 96-well test plate containing 100 μL of Luciferase Assay Reagent II (LAR II). The relative light units (RLU) were detected with a Thermo Scientific Fluoroskan Ascent FL (Thermo Fisher, USA).

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