PK-15, ST and 3D4/21 cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco, Grand Island, NY, USA) supplemented with 10% (v/v) foetal bovine serum (FBS; Gibco) at 37 °C with 5% CO2. The PRV variant HN1201 (GenBank Accession No. KP722022) was a kind gift from Ke-gong Tian at the College of Veterinary Medicine, Henan Agricultural University [16].

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