Genomic DNA was extracted from 2.5 × 106 purified CD19+ lymphocytes by using the QIAamp DNA kit (Qiagen, Hilden, Germany). An Ion Ampliseq panel was designed targeting all coding sequences and the exon–intron boundary regions up to 25-bp of the splicing junctions of the gene TP53. For DNA library construction, two primer pools were designed by Ion AmpliSeq™ Designer v4.2 (Life Technologies, Foster City, CA, USA). Targeted NGS was performed by the Microarray Facility of the University of Ferrara using Ion Torrent PGM (Life technologies, Foster City, CA, USA), as previously described [19]. Sequencing results were compared to the human reference genome (GRCh37). Data analysis and variants identification were carried out by the Torrent Suite 3.4 and Variant Caller plugin 3.4.4 software (Life technologies). All data are available at the public archive European Variation Archive (EVA) with the accession number PRJEB34654.

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