A ceftaroline stock solution was prepared every day by dissolving ceftaroline dihydrochloride in a mixture of water:methanol (1:1; v:v) to obtain a concentration of 1 mg/mL. For the standard calibration samples, the stock solution was diluted with water to prepare the working solutions (400, 200, 100, 50, 10, 5 and 2.5 µg/mL). A total of 100 µL of every working solution was mixed with 900 µL of drug-free human plasma to obtain the standard calibration samples (40, 20, 10, 5, 1, 0.5 and 0.25 µg/mL).

Three QC samples (high, medium and low) were prepared. The stock solution was diluted with water to prepare the working solutions (300, 150 and 7.5 µg/mL), and 100 µL of every working solution was mixed with 900 µL of drug-free human plasma to obtain the QC samples (30, 15 and 0.75 µg/mL).

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