The chromatographic system used was a WatersTM 1525 binary HPLC pump connected to an in-line degasser, an autosampler (2707) and UV/visible detector (2498). The HPLC system was controlled with the Waters Breeze HPLC software (Version 6.20.00.00., Waters, Milford, MA, USA), which was also used to process the data. The final conditions of the method were selected from preliminary experiments, in which different columns, mobile phase composition, flow rate and sample preparation conditions were tested. A UV spectrum was used in order to select the wavelength for detection. Chromatographic analysis was performed using a Symmetry C18 (5 µm × 4.6 mm × 150 mm) column (Waters). The mobile phase for ceftaroline determination consisted of ammonium dihydrogen phosphate buffer:acetonitrile (85:15, v:v). Buffered solution was prepared by dissolving 575 mg of ammonium dihydrogen phosphate in 1000 mL of ultrapure water. Later it was filtered and degassed in an Ultrasons ultrasonic bath (Selecta, Barcelona, Spain) and delivered with a flow rate of 1 mL/min. The assay was performed at room temperature (RT) and the selected wavelength to detect ceftaroline was 238 nm.

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