Ceftaroline dihydrochloride was kindly supplied by Pfizer Inc. Acetonitrile HPLC gradient (ACN) and methanol were purchased from Scharlau (Barcelona, Spain), and ammonium dihydrogen phosphate was purchased from Sigma-Aldrich Chemie Gmbh (Steinheim, Germany). The ultrapure water was obtained from the Mili-Q® Plus apparatus (Millipore, Burlington, MA, USA).

Blank plasma from healthy donors, used to prepare calibration standard and quality control (QC) samples, was provided by the Basque Biobank (www.biobancovasco.org, accessed on 21 May 2021) and was processed following standard operation procedures with appropriate approval from the Ethical and Scientific Committees (Code CES-BIOEF 2020-34).

Plasma from critically ill patients was processed following a protocol previously approved by the Basque Clinical Research Ethics Committee (EPA2018019 (SP)). Samples and data from these patients were provided by the Basque Biobank (www.biobancovasco.org, accessed on 21 May 2021) and were processed following standard operation procedures with appropriate ethical approval.

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