The blood samples were collected in three areas where malaria is endemic in the Republic of Korea (ROK) (n = 11), Thailand (n = 7), and Myanmar (n = 12) from 2014 to 2016, 2013, and 2012, respectively. The healthy individual serum samples were collected from children under 10 years old with no malaria history in local hospitals of areas where malaria is not endemic in the ROK. For the determination of immunoglobulin G (IgG) levels, a total of 112 serum samples were collected from malaria febrile patients with symptoms and positive P. vivax density by microscopic examination (40,000 to 260,000 parasites/μl) from Tengchong county, an area at the China-Myanmar border of the Yunnan province in 2016. In addition, 80 serum samples of healthy individuals that tested negative for P. vivax malaria by microscopy were collected in areas of China where malaria is not endemic (Wuxi city, Jiangsu province). The study was approved by the Ethics Committee of the National Institute of Parasitic Diseases (NIPD), China CDC.

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