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The NPY action on POMC neurons was analyzed under voltage clamp. Based on previous studies5658 and on the peptide-induced action-potential frequency modulation, which we observed in current-clamp recordings, the holding potential was set to −55 mV to optimize the recording conditions to measure inward currents. NPY (100 nM) was bath applied for 10 min after a 5-min baseline recording. The recorded peptide-induced currents were baseline subtracted and the mean (±s.e.m.) was calculated. To quantify differences in the peptide-induced currents, the area under the curve (electrical charge in nC) during the 10-min application of NPY was calculated.

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