Participants attended the clinic in the morning after an overnight fast (minimum 8 hours) and blood samples were taken on arrival. Triglyceride and high-density lipoprotein cholesterol (HDL-C) levels were measured on Olympus 5400 biochemistry analysers with Olympus reagents using standardised procedures and fresh samples (Olympus Diagnostica GmbH, Hamburg, Germany). Abnormal metabolic factors were defined as high triglycerides ≥1.7mmol/l and low HDL-C (<1.03 mmol/l in males or <1.29 mmol/l in females) [41].

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