Also in the Article



We used publicly available single-cell RNA-seq data from mouse hypothalami to detect overlaps with markers identified in our Glp1r and Lepr datasets. The first data source used was single-cell RNA-seq of 20,921 cells from the arcuate–median eminence complex of mice11. We filtered cells expressing POMC (4,248/20,921 cells) and clustered them using the R Seurat package24. The clustering result was visualized using the UMAP plot of Seurat. The second data source used was from the single-cell RNA-seq dataset of 163 POMC-expressing neurons in mice yielding four clusters12.

Note: The content above has been extracted from a research article, so it may not display correctly.



Also in the Article

Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.