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For POMCDre LeprCre ROSA26lSlrSrEGFPL10a and POMCDre Glp1rCre ROSA26lSlrSrEGFPL10a animals, 6 triple transgenic bacTRAP mice (3 females and 3 males) and 12 triple transgenic bacTRAP mice (6 females and 6 males) were pooled for each replicate, respectively, accounting for three to four replicates per POMC subpopulation. Mice were killed at 12 weeks of age in a random-fed state by decapitation. Whole hypothalami were obtained using a mouse brain slicer matrix and snap frozen in liquid nitrogen until translating ribosome affinity purification (TRAP).

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