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Glucose tolerance tests were performed at 13 weeks of age with 16-h fasted mice. Body weights of mice and their basal blood glucose using a glucometer and glucose strips (Contour Next, Bayer HealthCare) were determined before the start of the experiment. Mice were injected with 20% glucose, and blood glucose was measured at 15, 30, 60 and 120 min after injection.

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