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Lean and fat mass were determined via nuclear magnetic resonance (NMR Analyzer Minispec mq 7.5, Bruker Optik) in live mice. Alternatively, body composition was analyzed by computed tomography (CT) in isoflurane-anesthetized mice (Dräger and Piramal Healthcare). For data acquisition on an IVIS Spectrum CT scanner (Caliper LifeScience), we used IVIS LivingImage Software V.4.3.1. Quantification of lean and fat mass contents were determined with a modification of the previously described VINCI software package 4.61.0.

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