To assess association between the histological type of FTs and gender, the Cochran–Armitage test was used. The Jonckheere–Terpstra test was used to assess associations between the histological type of FTs and differences in the pattern of 53BP1 expression. This statistical test is a rank-based nonparametric test that can be used to determine if there is a statistically significant trend between an ordinal independent variable (the pattern of 53BP1 expression) and a continuous or ordinal dependent variable (the histological type of FTs, such as AG< FA< FT-UMP< FC). Differences in the frequency of the abnormal type of 53BP1 expression between FA and FC were evaluated using Student’s t-test. Differences in the frequency of each type of 53BP1 expression between gender were also evaluated using Student’s t-test. The significance of 53BP1 expression by IF as a diagnostic test was evaluated by logistic regression model and receiver operating characteristic (ROC) curve. All statistical analyses were performed using SAS software (version 9.4, SAS Institute Inc., Cary, NC, USA) with statistical significance set at values less than 0.05.

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