RNA-Seq based analysis of 78 tumors from the West Midlands Bladder Cancer Prognosis Programme (BCPP, ethics approval 06/MRE04/65). All ethical regulations for work with human participants were followed. RNA libraries were prepared using the Truseq Stranded Total RNA with Ribo-zero Gold kit (Illumina) and 2 × 100 bp PE sequenced (Hiseq, n = 26) or 2 × 75 bp PE sequenced (Nextseq, n = 52). The data were aligned to GRCh37 and reads counted with STAR aligner (v2.5.2b). Log2(Read count+1) for each gene has been used as input for the class prediction.

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