To assess extraction efficiency, we created five pools of urine samples and spiked 237.5 µL of each pooled sample with 12.5 µL of three different concentrations of an OT standard (high = 4000 pg/mL; medium = 2000 pg/mL; low = 1000 pg/mL) preceding extraction. Furthermore, to assess assay accuracy, 237.5 µL of each pooled sample were spiked with 12.5 µL of three different concentrations of an OT standard (high = 4000 pg/mL; medium = 2000 pg/mL; low = 1000 pg/mL) following extraction. Values from the spiked and unspiked samples were used to calculate percent recovery for extraction efficiency and assay accuracy (28, 30).

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.