Vero E6 cells (20,000 cells/well) were seeded in 96-well clear flat-bottom plates and incubated at 37 °C with 5% CO2 for 24 h. After incubation, Pelargonidin at concentration of 20, 50 and 100 μM, and Remdesivir 10 µM (Veklury, Gilead, US) all diluted in complete medium were added to each well and then, cells were infected with SARS-CoV-2 (50 or 100 TCID50/well). Negative controls (pelargonidin or Remdesivir alone), infected positive controls (SARS-CoV-2 alone) and mock-infected cells were included in each plate. Plates were incubated at 37 °C with 5% CO2 for 48 h. After the incubation, supernatants of 5 technical replicates were pooled and stored at −80 °C for further analysis.

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