The experiments were performed in Biological Safety Level 3 (BSL-3) virology laboratory of “Santa Maria della Misericordia” Hospital, Perugia, Italy. The SARS-CoV-2 strain was isolated as previously described [30]. For viral propagation, Vero E6 cell line was maintained in Eagle minimal essential medium (MEM) supplemented with 10% foetal bovine serum (FBS) and 100 U/mL penicillin–streptomycin solution. After isolation, SARS-CoV-2 titer was determined by Median Tissue Culture Infectious Dose (TCID50) endpoint dilution and stock aliquots were stored at −80 °C. The stock virus titer was 4.22 × 107 TCID50/mL.

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