The proteins from NCMECs were fractionated by electrophoresis on 10% SDS-PAGE, and incubated with the primary antibody followed with a horseradish peroxidase-conjugated secondary antibody. The experiment was repeated three times. The antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used to verify equal loading of proteins. Densitometry was performed with ImageJ software.

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