The proteins from NCMECs were fractionated by electrophoresis on 10% SDS-PAGE, and incubated with the primary antibody followed with a horseradish peroxidase-conjugated secondary antibody. The experiment was repeated three times. The antibody to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used to verify equal loading of proteins. Densitometry was performed with ImageJ software.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.