NCMECs were fixed in 10% neutral buffered formalin; then washed with PBS and treated with 0.2% Triton X-100 for 10 min; then TUNEL reaction mixtures were added and incubated for 1 h at 37°C after blocked with normal horse serum for 30 min. Cell nuclei were stained with DAPI (ZLI-9557; ZSGB-BIO, Beijing, China). Photographs were obtained using an Olympus BX63 fluorescence microscope (Olympus America Inc., Center Valley, PA, USA) at 10× magnification. The number of TUNEL-positive cells and total cells were counted in each field using ImageJ software. The percentage of TUNEL-positive cells to total cells was calculated.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.