Heart slices of all animal models were first immersed in Bouin’s fixative for 30 min and then washed with tap water, then the slices were immersed in Sirius Red solution (Direct Red 80 and saturated picric acid, Sigma) and briefly washed in 0.5% acetic acid (Thermo Fisher Scientific). Finally the slices were mounted with a xylene-based mounting media (Richard-Allen Scientific, Kalamazoo, MI, USA). The photos were scanned with a digital slide scanner (Pannoramic SCAN, 3DHISTECH).

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