For quantifying the mRNA expression of genes, cells were seeded in six-well plates. After 48 h of the transfection, cells were harvested and total RNA was isolated using a HP Total RNA Kit (Omega, Norcross, GA, USA) according to the manufacturer’s protocol. The cDNA was synthesized using a PrimeScript ™ RT reagent Kit with gDNA Eraser (Takara) according to the manufacturer’s protocol. The qRT-PCR was performed in triplicate with iQSYBR green Supermix (Bio-Rad) in a LightCycler480 Realtime PCR machine (Roche). The mRNA levels of target genes were reported relative to those of the house keeping gene β-actin by using the 2−∆∆Ct method. The qRT-PCR primers are listed in Supplementary Table 1 (see section on supplementary materials given at the end of this article).

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