RNA extraction and qRT-PCR
This protocol is extracted from research article:
Novel adipokine asprosin modulates browning and adipogenesis in white adipose tissue
J Endocrinol, Mar 9, 2021; DOI: 10.1530/JOE-20-0503

Total RNA was extracted from cells or tissues with the use of TRIzol reagent (D9108A, Takara Bio). RNA was reverse-transcribed using the RNA PCR Kit (RR036A, Takara Bio). Quantitative PCR (qPCR) amplification was performed with an ABI PRISM 7900 Sequence Detector system (Applied Biosystem, Foster City, CA) according to the manufacturer’s instructions. Relative gene expression (normalized to 18S) was calculated using the comparative CT method formula 2-∆∆CT. The real-time PCR primer sequences are shown in Supplementary Table 1 (see section on supplementary materials given at the end of this article).

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