Animals
Generation of recombinant adenovirus
Metabolic analysis
Enzyme-linked immunosorbent assay
RNA extraction and qRT-PCR
Histological analysis
MEF culture, differentiation, and treatment
Red Oil O staining
Statistical analysis
Cells or tissues were homogenized in ice-cold RIPA buffer with a proteinase inhibitor cocktail (Sigma-Aldrich). Protein concentrations were determined using the BCA Protein assay kit (Thermo Scientific). Equal amounts of protein were fractionated by 10% SDS polyacrylamide gels, followed by immunoblotting with the following primary antibodies: asprosin (FNab09797), UCP1 (ab10983; Abcam), PGC1a (ab54481; Abcam), β-tubulin (ab6046; Abcam). Membranes were then incubated with peroxidase-conjugated secondary antibody, and specific bands were detected with a Bio-Rad (Hercules, CA) imaging system.