Enzyme-linked immunosorbent assay
This protocol is extracted from research article:
Novel adipokine asprosin modulates browning and adipogenesis in white adipose tissue
J Endocrinol, Mar 9, 2021; DOI: 10.1530/JOE-20-0503

Enzyme-linked immunosorbent assay (ELISA) was performed to measure asprosin levels in mice serum by using commercial kits (Bioswamp, MU30851) according to the manufacturer’s instruction. Read the absorbance at 450 nm immediately after adding the stop solution. Quantitative results were calculated by standard curves.

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