The cytokines IFN-γ and IL-2 were measured in cell culture supernatants using human or mouse Milliplex MAP Kits (HCYTMAG-60K, MCYTMAG-70K, Millipore, Billerica, MA, USA), according to the manufacturer’s instructions. Quantification was performed with a Magpix instrument and xPONENT 4.2 or 4.3 software (Luminex Corp., Austin, TX), or Bio-Plex 200® System and Bio-Plex Manager software version 5.0 (BIO-RAD Laboratories, Hercules, CA, USA). The concentration of each cytokine was determined from an 8-point standard curve using five parameter logistic regression. The samples below minimum detectable concentration (MinDC) were given an arbitrary value of 50% of MinDC. Each sample was compared to an unstimulated sample.

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