15-mer peptides covering hemagglutinin (GenBank entry ACP41953.1) and neuraminidase (YP_009118627.1) of influenza (A/California/07/2009 (H1N1)), and nucleoprotein (ADE29096.1) of influenza (A/reassortant/NYMC X-179A (California/07/2009 × NYMC X-157)(H1N1)) with 12 amino acid overlap were produced. 2 × 5 15-mer peptides with 12 amino acid overlap, covering 2 viral peptide mimotopes identified in POMT1 (AAH65268.1) and SNTG1 (AAI04830.1), respectively, were also produced (all peptides from New England Peptides, Gardner, MA, USA). Peptides that failed in-house quality control by mass spectrometry were excluded (0–5.4% of viral peptides). Peptides were dissolved in endotoxin-free water/DMSO (50%; both from Sigma-Aldrich, Schnelldorf, Germany), and stored as 25 mM stock solutions at −70 °C, until the day of use. Recombinant influenza A H1N1 (A/California/07/2009) hemagglutinin (cat#11085-V08H), (A/California/04/2009) neuraminidase (cat#11058-VNAHC or cat#11058-V01H), and (A/California/07/2009) or (A/Puerto Rico/8/34/Mount Sinai) nucleoprotein (cat#40205-V08B and cat#11675-V08B) were purchased from Sino Biological, Beijing, China. Endotoxin-free, recombinant human protein-O-mannosyltransferase 1 and syntrophin gamma 1 were from Origene, Rockville, Maryland, and endotoxin-free ovalbumin from Hyglos, Bernried, Germany.

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