Cells were stained with NUCLEAR-ID Red DNA stain (30 min, 37 °C, 5% CO2), washed and mounted with Dako Fluorescence Mounting Medium (ENZO, Farmingdale, NY, USA). Images were acquired on a Zeiss upright fluorescence Axio Scope.A1 microscope and analysed using ImageJ. In each replicate, at least nine randomly chosen images were taken and number of nuclei quantified after setting the threshold automatically, using Li filter and ‘Analyse particle’ function in Batch mode.

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