Plastic coverslips (Fisher) were placed in the diluted culture in a six well plate and allowed to grow 48 h at room temperature. Antibiotics were added at 48 h, and biofilms were allowed to grow another 72 h before being fixed in 4% PFA and stained with SYTO 9 (ThermoFisher). Images were taken using a Zeiss 710 confocal laser scanning microscope. To obtain a representative sample of the biofilms, at least three images were taken along the air-liquid interface from at least five biological replicates.

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