Total RNAs were extracted using the RNeasy Plus Mini Kit (Qiagen) and cDNA was prepared using the SuperScript VILO cDNA Synthesis Kit (Thermo Fisher Scientific) according to manufacturer's protocols. qRT-PCR was performed using the StepOne Real-Time PCR System and PowerTrack SYBR Green Master Mix (Thermo Fisher Scientific) following the instructions. Data were examined by the 2-ΔΔCt method. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a housekeeping gene. Primers for qRT-PCR are listed in Supplementary Table S1.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.