All small oligomers were passed through a Superdex200 Increase 10/300 GL column (Cytiva) while larger assemblies were passed through a Superose 6 Increase 10/300 GL column (Cytiva) on a AKTA PURE FPLC system. The mobile phase was TBS (TBS, 25 mM Tris, 300 mM NaCl). In addition, for the icosahedral assembly, an additional custom packed 10/300 Sephacryl500 column (Cytiva) was used to separate out the void. Samples were run at a speed of 0.75 mL/min and eluted with 0.5 mL fractions.

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