The hanging drops technique according to Insphero (InSphero AG) manufacturer’s instructions was used. Single DHSF-BR16 and MCF-7 cells were seeded at various cell densities (ranging from 1000 to 8000) into special hanging drop culture plates. In order to obtain optimal spheroids (200–300 µm) without central necrotic area after 4 days of culture, an inoculum of 2000 cells/well was used. MCF-7 culture conditions are described in Supplementary Methods.

Note: The content above has been extracted from a research article, so it may not display correctly.

Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.

We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.