The hanging drops technique according to Insphero (InSphero AG) manufacturer’s instructions was used. Single DHSF-BR16 and MCF-7 cells were seeded at various cell densities (ranging from 1000 to 8000) into special hanging drop culture plates. In order to obtain optimal spheroids (200–300 µm) without central necrotic area after 4 days of culture, an inoculum of 2000 cells/well was used. MCF-7 culture conditions are described in Supplementary Methods.

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