Mature polar cod were captured south of Northeast Land, Svalbard (78°55′N, 23°40′E) using a bottom trawl fitted with a live fish box90 aboard the RV Helmer Hanssen in November of 2017. Fish were kept on deck in four 400L flow-through seawater tanks during the 8-day transport to the Aquaculture Research Station in Tromsø, Norway. Broodfish were acclimated to the laboratory in a 4000L tank under a daylight cycle of 80°N at 2.3–2.5 °C, fed to satiation with frozen wild Calanus zooplankton (Calanus AS) and treated with Halamid disinfectant solution (0.01 g/L) as needed. Experimental timing was based on the gravid status of adults, as determined from daily pilot strip spawns of anesthetized females.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.