Mature polar cod were captured south of Northeast Land, Svalbard (78°55′N, 23°40′E) using a bottom trawl fitted with a live fish box90 aboard the RV Helmer Hanssen in November of 2017. Fish were kept on deck in four 400L flow-through seawater tanks during the 8-day transport to the Aquaculture Research Station in Tromsø, Norway. Broodfish were acclimated to the laboratory in a 4000L tank under a daylight cycle of 80°N at 2.3–2.5 °C, fed to satiation with frozen wild Calanus zooplankton (Calanus AS) and treated with Halamid disinfectant solution (0.01 g/L) as needed. Experimental timing was based on the gravid status of adults, as determined from daily pilot strip spawns of anesthetized females.

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