To evaluate the DNA index, unfixed DHSF-BR16 cells and human lymphocytes obtained from a health volunteer were stained with a Propidium Iodide (PI) staining technique15. The DNA index was calculated as the ratio of DHSF-BR16 cells and peripheral blood diploid (2n) lymphocytes G0/G1 fluorescence channels. According to Ross et al.16, a DNA index of 1.0 is indicative of diploidy whereas a ratio ranging from 0.85 to 1.9 is indicative of ipo- or hyper-diploidy, respectively.

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