Genomic DNA extraction from mouse tails was conducted using the standard ethanol precipitation protocol. The tissue digestion solution contains 1 mg/mL of proteinase K, 50 mM Tris-HCl (pH = 8.0), 100 mM EDTA, 100 mM NaCl, and 1% SDS. Genotyping was performed with PCR-based assays using purified genomic DNA and primer pairs to detect Rax-CreERT2, GFAP-CreERT2, iDTR, iDTA, Igf1rf/f, BrafV600E, and Ai14 alleles, respectively. The primers used for genotyping are listed in Supplementary Table 1.

Note: The content above has been extracted from a research article, so it may not display correctly.



Q&A
Please log in to submit your questions online.
Your question will be posted on the Bio-101 website. We will send your questions to the authors of this protocol and Bio-protocol community members who are experienced with this method. you will be informed using the email address associated with your Bio-protocol account.



We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.