All experimental mice were under the husbandry care of the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. The mouse strains including Rax-CreERT2 (strain name: Raxtm1.1(cre/ERT2)Sbls/J; stock number: 025521), Igf1rf/f (strain name: B6;129-Igf1rtm2Arge/J; stock number: 012251), BrafV600E (strain name: Braftm1Mmcm/J; stock number: 017837), Ai14 (strain name: B6.Cg-Gt(ROSA)26Sortm9(CAG-tdTomato)Hze/J, stock number: 007914), iDTA (strain name: B6;129-Gt(ROSA)26Sortm1(DTA)Mrc/J, stock number: 010527), and GFAP-CreERT2 (strain name: B6.Cg-Tg(GFAP-cre/ERT2)505Fmv/J, stock number: 012849) were obtained from the Jackson Laboratory. Inducible DTR mouse line (strain name: B6-Gt(ROSA)26Sortm2(CAG-LSL-DTR-EGFP)) was generated by Biocytogen. Two-month-old wild-type C57BL/6N mice and 5-week-old BALB/c-nu mice were ordered from SPF Biotechnology Co. Ltd and housed in the same animal facility on a 12-h reverse light/dark cycle and provided with food and water ad libitum. The animal facility was maintained at a temperature of 21 °C with 50–60% humidity. All mice in the study were backcrossed to the C57BL/6N background for at least six generations. To perform lineage tracing, targeted neural injury and cell-type-specific genetic manipulation, we crossed Rax-CreERT2 driver mice with Ai14, iDTR, Igf1rf/f, or BrafV600E mice during adulthood to generate corresponding mouse lines. All procedures, husbandry, and experiments were performed according to the policies and ethical regulations established by the Institutional Animal Care and Use Committee at the Institute of Genetics and Developmental Biology, Chinese Academy of Sciences. The experimental protocols used in this study received ethical approval from the Chinese Academy of Sciences.

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