For quantitative reverse transcription PCR (RT-qPCR) of antiviral genes, total cellular RNA was reverse transcribed using the Maxima H Minus First Strand cDNA Synthesis Kit with random primers (ThermoFisher). cDNA was used for qPCR using specific primers (Supplementary Table 3) and the Power SYBR Green PCR Master Mixture (Applied Biosystems) on a Step-One-Plus Real-Time PCR thermocycler (Applied Biosystems). The relative fold gene expression of genes was calculated relative to GAPDH using the delta-delta Ct method, comparing to mock conditions.

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