AB strain zebrafish were handled as previously described49. The zebrafish study was approved by Animal Care and Use Committee of Jeju National University (Jeju Special Self-governing Province, Republic of Korea; approval No.: 2020-0013). All methods were carried out in accordance with relevant guidelines and regulations. Additionally, all the methods were carried out in accordance with the ARRIVE guidelines50. Zebrafish were raised at 28.5℃ with a 14:10-h light:dark cycle in a water-recirculating tank system (pH 7.4 and 0.03% salinity). Fertilized embryos were collected after natural spawning and cultured at 28.5 °C in E3 embryo media containing 2 mg/L methylene blue. To inhibit melanin formation, 0.003% PTU was added to the egg water throughout the experimental period.

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