Total RNA was isolated from RAW 264.7 macrophages using easy-BLUE total RNA Extraction Kit (iNtRON Biotechnology, Seongnam, Gyeonggido, Republic of Korea) according to the manufacturer’s instruction. The RNA was reverse-transcribed by Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase Kit (Bioneer, Daejeon, Republic of Korea). In brief, synthetic cDNA was amplified using specific primers44,46.

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