Virus isolation was performed on lung tissues by homogenizing the tissue in 1 mL DMEM using a TissueLyser (Qiagen) and inoculating Vero E6 cells in a 96-well plate with 200 µL of a 1:10 dilution series of the cleared homogenate. One hour after inoculation of cells, the inoculum was removed and replaced with 200 µL DMEM (Sigma-Aldrich) supplemented with 2% fetal bovine serum, 1 mM l-glutamine, 50 U/mL penicillin and 50 µg/mL streptomycin. Six days after inoculation, cytopathogenic effect was scored and the TCID50 was calculated using the Reed-Muench method43.

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