RAW 264.7 macrophages were obtained from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in DMEM supplemented with 5% FBS at 37℃ in 5% CO2. For the analysis of cell viability, the cells were seeded at a density 1 × 105 cells/mL in 24 well plate and incubated with the indicated concentrations of fisetin. After 24-h incubation, MTT assay was performed.

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