Work with infectious SARS-CoV-2 complied with all relevant ethical regulations for animal testing and research. The hamster study received ethical approval from the Rocky Mountain Laboratories Animal Care and Use Committee (IACUC, Protocol # 2020-044-E) and was performed in a high biocontainment laboratory at Rocky Mountain Laboratories (RML), NIAID, NIH. Animal work was performed by certified staff in an Association for Assessment and Accreditation of Laboratory Animal Care International accredited facility. Work followed the institution’s guidelines for animal use, the guidelines and basic principles in the NIH Guide for the Care and Use of Laboratory Animals, the Animal Welfare Act, United States Department of Agriculture and the United States Public Health Service Policy on Humane Care and Use of Laboratory Animals.

Syrian hamsters were group housed in HEPA-filtered cage systems enriched with nesting material and were provided with commercial chow and water ad libitum. Animals were monitored at least twice daily throughout the study.

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