After weaning, female mice from the parental generation were anesthetized with isoflurane and euthanized by decapitation. Trunk blood samples were rapidly collected in autoclaved tubes, and the serum was separated by centrifugation (3000 rpm for 30 min). Proinflammatory cytokines IL-1β, IL-10, IL-17, IL-23, and TNF-α were detected using a LEGENDplex Mouse Inflammation Panel (Biolegend, CA, USA).

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