The T-maze test was performed using a T shaped maze, 30 inches elevated from the ground. The total length of the top arm was 44 inches and the bottom arm was 20 inches. All arms had walls. For the test, a single mouse was placed in the outermost corner of the bottom arm and was allowed to explore the whole maze for 5 min while videotaping the session. Working memory was evaluated by quantifying the number of spontaneous alternations between entering the three different arms25.

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