The EPM test was performed using a four-arm plus shaped maze elevated 30 inches from the ground, with two arms with walls and two arms open. The arm length was ~ 20 inches with a 4-inch center square between all four arms. Individual mice were placed in the center part and then were allowed to freely explore for 5 min while the session was videotaped. The time the mouse spent in the closed arms versus the open arms was quantified for detecting avoidance behavior23.

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