The open field test was done using a large plastic container measuring 12in width × 18in length × 12in height. Individual mice were placed inside and allowed to freely explore the arena for 5 min. The session was videotaped and then analyzed using Ethovision XT tracking system, which was used to analyze all behavioral tests (Noldus, Leesburg, VA). Several parameters were quantified: Total distance traveled, speed of movement and time spent in the center versus periphery regions. Additionally, the number of rearing events and cumulative grooming time were measured. This test was done on both parents and offspring.

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