Total thymocytes were plated at a density of 1 × 106 ml−1 and incubated at 37 °C for 4 h in the presence of Cell Stimulation Cocktail (eBioscience, 00-4975-93) and Protein Transport Inhibitor Cocktail (eBioscience, 00-4980-93) in RPMI 1640/10% fetal bovine serum followed by cytokine detection by intracellular staining.

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